| 质粒名称: | pDD04neo pmyo2::gfp |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26393 |
| 存储实验室: | Denis Dupuy |
|
载体骨架基本信息 |
|
| 载体名称: | pBS |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，蠕虫表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | C. elegans (nematode) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pDD04neo pmyo2::gfp was a gift from Denis Dupuy (Addgene plasmid # 26393 ; http://n2t.net/addgene:26393 ; RRID:Addgene_26393) |
| 参考文献部分: | An antibiotic selection marker for nematode transgenesis. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1494 PubMed 20729841 |
买家导航
pDD04neo pmyo2::gfp载体质粒基本信息
pDD04neo pmyo2::gfp质粒图谱载体图谱和pDD04neo pmyo2::gfp载体序列质粒序列多克隆位点信息
pDD04neo pmyo2::gfp质粒载体简介
pDD04neo pmyo2::gfp质粒序列载体序列
LOCUS pDD04neo pmyo2::gfp 9672 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pDD04neo pmyo2::gfp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9672)
AUTHORS Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R,
Baillie D, Vidal M, Dupuy D
TITLE An antibiotic selection marker for nematode transgenesis.
JOURNAL Nat Methods. 2010 Aug 22. ():.
PUBMED 20729841
REFERENCE 2 (bases 1 to 9672)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9672
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 24..128
/gene="bla"
/label=AmpR promoter
CDS 129..989
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(347..366)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1160..1748
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1649..1668
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1902..1919
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2036..2057
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2072..2102
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2110..2126
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2115..2137
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2134..2150
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2134..2150
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 2169..2189
/label=T3
/note="T3 promoter, forward primer"
promoter 2171..2189
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 2226..2242
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2226..2242
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(2276..2292)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2277..2293)
/label=pBluescriptKS
/note="For pBluescript vector"
CDS complement(3001..3795)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3112..3131)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 3722..3741
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
protein_bind 4632..4652
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
protein_bind 7165..7189
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(7214..7242)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 7990..8010
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
protein_bind complement(8942..8966)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(9028..9047)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(9029..9047)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 9215..9670
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9302..9321)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9512..9533
/label=F1ori-F
/note="F1 origin, forward primer"
ORIGIN
1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
2221 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata
2281 ccgtcgacct cgagaaacag ttatgtttgg tatattggga atgtattctg tcatttaagg
2341 caaaaacccc atagacacta ctccactttc aaattatcaa cttctactca ccttccactg
2401 agcctcaaac ccaaaccttc ttccgatctt tcttgcatcg tgctcatcaa tacttgtgat
2461 tttagcaaga aaaacgccgt cctcgaaact cttgacaaac aataggggaa acccccaaaa
2521 agcaaaagca ggaaaattaa tttcataaac tttcacttga ttgaaaaatt ccaaaaatac
2581 tccaaaactt tttcacgatg agcatgattt gacgtcatga gaggcccaga cgtgcgaaga
2641 aataaaaatt gcggtcataa actgaaacgt aacatatgat aaggtatttt gtgtgcggtt
2701 ttttctatga tgtttcaaaa aaaatttatc agaagtaaaa aaaacataag aagtgtacac
2761 agaacattgt gtagattttg ggatagaatt attattaatg gggggaggga gcacaatttt
2821 tttcgacttt ttattacgaa ttaattctat ttttgaatct acacaatttc attgttagag
2881 gtgacttaaa agaagctaaa aaacaaagaa attaagaaga agtttttttg ataataacaa
2941 aaataggggg tgggagcaca gggagaaaga gcatgtaggg atgttgaaga gtaattggac
3001 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
3061 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
3121 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
3181 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
3241 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
3301 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
3361 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
3421 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
3481 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
3541 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
3601 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
3661 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
3721 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
3781 atcttgttca atcattttta ttccacttgt tgagcggggc tggctgaaaa tatgtgaatt
3841 gagaaaataa atagtatatt tgaaagaagg gaagacgaga taaacggaaa aacacgattg
3901 cggaacatta cgaaaaattt cgaaatttat tattttttta ataacatttg tttaccggcc
3961 ggacaagaca aaccaacttc gaaacgagga ataaatcaaa tttcatattt aaaatgaatc
4021 aaaacccatg attaaacgaa atgacaaaaa tcgataacgt gtatagaacg aactcaagct
4081 gaaccgagaa ccgagaaatt cagggttttt agtattttat tatttatttt agttttattt
4141 aagctgataa tttcgaaacg ttatgatttg aataaaaatc gtggaatatt gatattttcc
4201 caaaaattcc acttggtaga atcacaaatc taggcaaatt ggccgttttc tatcacaaat
4261 tacgagcgaa aactacaaat aatgcaattt ttatatactt ttctaactaa aaacctgaaa
4321 aaaatatcga aaaaatcggg aaaacagttg aaaaacggga aaaaaagggt taaaatttta
4381 ggcccagcaa tttcatcgcc ttccgcaaac cgtgcggtgg ggcggagaat gtaatggtgc
4441 ggcgcggttt tcggagtgtc gtagcgcgct gtgtttatcg taaatcggcg cgaactcttt
4501 tttgaagcaa ttttttttgg tttttattga aagttagtct caaaactcgt attattttaa
4561 aattttttta ttaaactaaa atttagaaat gggcccttgg atcctctaga gcggccgccg
4621 actagtgata tcaactttgt atagaaaagt tgaaggtctc ccagaacgtg aataaaagct
4681 gctcggacgt tgatgttatc gccgttgcca ccaccatgag agtgaccatg agagcttccg
4741 cctccgtgag aatgtgaatg accaccaaag tacaacaatg caagcatgct taaattttga
4801 ttttgaatat caattaagat acatatgtca cattagattt gtttcgaact caaaaaaatt
4861 tctttgaaaa ccgtaagtcc gtgacagcct aatttttagt taaaagaaaa taacatggac
4921 agacattttt ctgacatgtc aacattcctg gggctcccac ccggataaaa gttcatgcca
4981 aatatcgatt tcaaatcgaa gtaaaaaagt gtataccagt taataaagct atcagactcg
5041 tttcataaaa acacttacac caagtttaca accactccaa gagcggctgt caaagccata
5101 attcctcctt ctacttcata atctccgctg acaattctca taattgcgag aacaactaat
5161 actccagtga ctatccaaat aaggaaaact gagaaaaatg ctcccaaaac ttctgaaatt
5221 catttcaaac tatgtcgctc aattcttcca actacccacc ggctctatga aacccaaagc
5281 tcattttttg cgatggtggc cgtcttgcaa tgtacagaga aaacagcgaa atgagcactg
5341 acgcaaaatc agtgagcaag tgtgcggcat ctgtgacaat tgcgagtgag cccgcgagaa
5401 caccaccaat aacttcgcaa accataaaaa ataggcagag cacgacggtg agccataaaa
5461 ttcgcgttgc tgaaacaaat agtttgaaga aggtttcatt aggactcgag tttggcagaa
5521 attttaaaag caatacctct ccgattgcta tcatgtgaat ctgttgagtc agcttcatcg
5581 tggcaatgaa agttatcata ggtgactgtg gtttttctac cttcgtctgt attataaatg
5641 tgaggaactc tgtaatacaa aaagatctca tgttttttct ttggtaatac gtcatagaag
5701 cacaggaaat cacaactctg atataaacga acatcaacta catcacaaaa ctaacccatt
5761 ttcaagtagt ggtgctcgtt cagaaatcat tatggattat ggagaaaagg aaaccgatta
5821 ggccaatcga ttttgttttg atagaattga agacgcagaa taattgtgtt gtgtatagtg
5881 tacgagaaaa tggagcaact tgtatatggg cactgttatc attattgaat agcgtgcgga
5941 ggtttagaga aggaagttgt gtgttatcaa tcataaacta gaacatgtct cgaggcattt
6001 gaattggggg tggtggacag taactgtctg taataataat tactcctgac caggttgcaa
6061 ttcgagtttt gataagcata attatacctt gtacattgtg ggttttgtgc tgtggacgtt
6121 ttattgtgac atccccataa gctacaagaa caaaaatgaa atgtaaaagt attgaaaacg
6181 tcgtacattt tatatctgag tagtatcctt tgctttaaat gtccataaaa ctaattttat
6241 aatcaataaa acaacgtttg taaatcaact gagtttacaa gtagagacat tgagggatac
6301 tttcactatg ctaaagtgat aatcgaccaa ataataactt cactttggta tttattcctg
6361 tcttataaat gttatgtatg aattaaattc atatgcatat ggctcactct gacaattttt
6421 aataatcttc cagatcaata ttgactaccg atgcgggtgg tcttttgctt tgaattctgc
6481 tgaactttac accccgaaca gcaatgtgtg cttcagccta aaaaaaagta agtgtgttaa
6541 tcagtgcccc cgattcttca ttttttgccc ctctctcccg tttcgtcggc aaaagaagag
6601 aaaataaaga taagtctcaa gataggttgg taatcgctaa agtggttgtg tggataagag
6661 tagcaaaatg gcaggaagag cactttgcgc gcacacactg tactcattgt tctggataaa
6721 attctctcgt tgtttgccgt cggatgtctg cctctctgca ttgagccggc ttcttcacta
6781 tctttagtta acctaaaatg ccgtttcttt tctcgtatcc ccactatccc gttgaggttc
6841 tctgctctct tcgctcccta ccgccagcga gcaactatcc gtgggggcgc cttgctcgga
6901 agatgggggg gaagaaagaa gatttttgct atttgcactt gagaaagaga cttttcctgc
6961 gtcgatggtt agagaacagt gtgcagacac ttttcagcta cctagaatta caattggata
7021 tccccgcctc ccaatccacc cacccaggga aaaagaaggg ctcgccgaaa atcaaagtta
7081 tctccaggct cgcgcatccc accgagcggt tgacttctct ccaccacttt tcattttaac
7141 cctcgatcgt cagacacaga aatgacaagt ttgtacaaaa aagcagagta aaggagaaga
7201 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
7261 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
7321 tatttgcact actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac
7381 taaccctgat tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca
7441 atgcttctcg agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc
7501 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
7561 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt
7621 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
7681 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
7741 catggcagac aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac
7801 taactaatct gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag
7861 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
7921 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
7981 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
8041 actatacaaa tagcattcgt agaattccaa ctgagcgccg gtcgctacca ttaccaactt
8101 gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac tgagttctac
8161 taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg acttctaagt
8221 ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt
8281 gttattatca aaaaaacttc ttcttaattt ctttgttttt tagcttcttt taagtcacct
8341 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
8401 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
8461 gtacacttct tatgtttttt ttacttctga taaatttttt ttgaaacatc atagaaaaaa
8521 ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc
8581 ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt
8641 atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aatgtccttt cttttgcttt
8701 ttgggggttt cccctattgt ttgtcaagag tttcgaggac ggcgtttttc ttgctaaaat
8761 cacaagtatt gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca
8821 gtggaaggtg agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc
8881 ttaaatgaca gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt
8941 aacccagctt tcttgtacaa agttgatatc tcgacccggg aattccggac cggtaccagc
9001 tttgggcccg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcgctg
9061 caacattttg ctgccggtca tgactgggaa aaccctggcg ttacccaact taatcgcctt
9121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
9181 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
9241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
9301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
9361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
9421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
9481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
9541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
9601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
9661 cttacaattt ag
//
